Table 1.

Primary antibodies used in immunostaining

AntigenHostDilutionSource
Apical
    NKCC2, bumetanide-sensitive Na+-K+-2Cl cotransporterRabbit1:500M.A. Knepper
    NCC, thiazide-sensitive Na+-Cl cotransporter (A857)Rabbit1:40,000M.A. Knepper
    NCC, thiazide-sensitive Na+-Cl cotransporterRabbit1:500S.C. Hebert
    ENaC, β subunit of amiloride-sensitive epithelial Na channel (L558)Rabbit1:500M.A. Knepper
    AQP2, vasopressin-sensitive water channel, Aquaporin 2Rabbit1:500M.A. Knepper
Apical and basolateral
    H+-ATPase (B2) proton-ATPaseRabbit1:500M.A. Knepper
Basolateral
    NCX, Na+-Ca2+ exchangerRabbit1:1000Swant, Bellinzona
    AQP3, Aquaporin 3Rabbit1:500M.A. Knepper
    Na+-K+-ATPaseRabbit1:500Upstate Biotechnology, Lake Placid, NY
    PMCA, plasma membrane Ca-ATPaseMouse1:1Sigma, St. Louis, MO
    Cytoplasmic and nuclear
    CaBP, calbindin-D 28kMouse1:10000Swant, Bellinzona
    CaBP, calbindin-D 28kRabbit1:5000Swant, Bellinzona