Table 1.

Effect of human serum albumin (HSA) plus antioxidants, protein kinase C (PKC) inhibitors or dyphenyleneiodonium (DPI) and rotenone on tubular cell viabilitya

Viable Cell Count by Trypan Blue Dye Exclusion (% of Control)Cell Viability by MTT Colorimetric Assay (% of Control)
a Human proximal tubular (HK-2) cells were treated with 1,3-dimethyl-2-thiourea (DMTU) (30 mM), pyrrolidine dithiocarbamate (PDTC) (100 μM), calphostin C (1 μM), GF109203X (2.5 μM), dyphenyleneiodonium (DPI) (10 μM), or rotenone (10 μM) in the presence of HSA (30 mg/ml). Results are mean ± SE (n=3).
Control100100
HSA94.8 ± 2.1105.2 ± 3.2
HSA + DMTU94.2 ± 10.5118.6 ± 5.3
HSA + PDTC103.1 ± 5.2100.6 ± 1.9
HSA + calphostin95.1 ± 4.39103.1 ± 5.3
HSA + GF109203X101.8 ± 1.2103.8 ± 12.7
HSA + DPI108.0 ± 2.493.6 ± 9.2
HSA + rotenone103.7 ± 6.5100.6 ± 3.5