Table 3.

Effect of antioxidants on H2O2 production in HK-2 cells exposed to protein overloada

H2O2 (nmol/106 cells)
SupernatantCells
a HK-2 cells were treated with DMTU (30 mM) or PDTC (100 μM) 1 h before and during 5-min incubation with HSA or IgG (30 mg/ml). Results are mean ± SE (n = 3).
b P < 0.01 versus HSA.
c P < 0.01 versus IgG.
d P < 0.01 versus control.
Control1.3 ± 0.11.2 ± 0.1
HSA4.8 ± 0.3d4.9 ± 0.4d
HSA + DMTU1.0 ± 0.2b1.3 ± 0.1b
HSA + PDTC1.5 ± 0.2b1.8 ± 0.2b
IgG4.1 ± 0.1d5.0 ± 0.4d
IgG + DMTU1.5 ± 0.1c1.8 ± 0.2c
IgG + PDTC1.2 ± 0.1c1.8 ± 0.2c