Table 2.

Primers used in this study

MutationSequenceAmplification Products (bp)EndonucleaseMutant Fragments (bp)
3243A→G5′-AGGACAAGAGAAATAAGGCC-3′294ApaI184 + 112
5′-CACGTTGGGGCCTTTGCGTA-3′
3271T→C5′-AGGACAAGAGAAATAAAGGC-3′170AflII140 + 30
5′-TAAGAAGAGGAATTGAACCTCTGACCTTAAA-3′
8344A→G5′-AGCCCACTGTAAAGCTAACT-3′167BglI134 + 30
5′-TTGGGGCATTTCACTGTAAAGCCGTGTTGGT-3′
8993T→G/C5′-GGCCATCCCCTTATGAGCGG-3′180HpaII157 + 23
5′-ATGTTAGGCGGTTAGGCGTAC-3′
Common deletion5′-ACTACCACCTACCTCCCTCACC-3′160
5′-GCTTGTCAGGGAGGTAGCGATG-3′