Table 1.

Various mutated constructs made by altering initiation codon of env and/or accessory genes in pNL4-3: ΔG/P-EGFP parental construct

Viral ConstructMutated Gene(s)Expressed Gene(s)Start ATG Mutated to
pNL4-3: ΔG/P-EGFPenv, vif, vpr, vpu, tat, rev, and nef
pNL4-3: ΔG/P-EGFP/ΔEnvenvvif, vpr, vpu, tat, rev, and nefaCg
pNL4-3: ΔG/P-EGFP/ΔVifvifenv, vpr, vpu, tat, rev, and nefTGA
pNL4-3: ΔG/P-EGFP/ΔVprvprenv, vif, vpu, tat, rev, and nefGtg
pNL4-3: ΔG/P-EGFP/ΔVpuvpuenv, vif, vpr, tat, rev, and nefTGA
pNL4-3: ΔG/P-EGFP/ΔNefnefenv, vif, vpr, vpu, tat, and revTGA
pNL4-3: ΔG/P-EGFP/ΔRevrevenv, vif, vpr, vpu, tat, and nefaCA
pNL4-3: ΔG/P-EGFP/Tat-Revenv, vif, vpr, vpu, and neftat and rev
pNL4-3: ΔG/P-EGFP/Tatenv, vif, vpr, vpu, rev, and neftat
pNL4-3: ΔG/P-EGFP/Tat-Nefenv, vif, vpr, vpu, and revtat and nef