Table 1.

Sequences of primers and Taqman probes for real-time quantitative PCRa

GeneForward PrimerReverse PrimerProbe
a PCR primers and fluorescent probes (5′-6-carboxyfluorescein- and 3′-6-carboxytetramethylrhodamine-labeled) were designed by using the computer program Primer Express (Applied Biosystems) and were purchased from Biolegio. HPRT, hypoxanthine-guanine phosphoribosyl transferase; ECaC1, epithelial Ca2+ channel 1; CaBP, calbindin; PMCA1b, plasma membrane Ca2+-ATPase; NCX1, Na+/Ca2+-exchanger; R, rat; M, mouse.
HPRTR5′-TATCAGACTGAAGAGCTACTGTAATGACC-3′5′-TTACCAGTGTCAATTATATCTTCAACAATC-3′5′-TGAGAGATCATCTCCACCAATAACTTTTATGTCCC-3′
M5′-TTATCAGACTGAAGAGCTACTGTAATGATC-3′5′-TTACCAGTGTCAATTATATCTTCAACAATC-3′5′-TGAGAGATCATCTCCACCAATAACTTTTATGTCCC-3′
ECaC1R5′-CTTACGGGTTGAACACCACCA-3′5′-TTGCAGAACCACAGAGCCTCTA-3′5′-TGCTTCTCAGATAGTTGTTCTTGTACTTCCTCCTTGT-3′
M5′-CGTTGGTTCTTACGGGTTGAAC-3′5′-GTTTGGAGAACCACAGAGCCTCTA-3′5′-TGTTTCTCAGATAGCTGCTCTTGTACTTCCTCTTTGT-3′
CaBP-D28kR5′-GGAAGCTGGAGCTGACAGAGAT-3′5′-TGAACTCTTTCCCACACATTTTGAT-3′5′-ACCAGTGCAGGAAAATTTCCTTCTTAAATTCCA-3′
M5′-AACTGACAGAGATGGCCAGGTTA-3′5′-TGAACTCTTTCCCACACATTTTGAT-3′5′-ACCAGTGCAGGAAAATTTCCTTCTTAAATTCCA-3′
CaBP-D9kM5′-CCTGCAGAAATGAAGAGCATTTT-3′5′-CTCCATCGCCATTCTTATCCA-3′5′-CAAAAATATGCAGCCAAGGAAGGCGA-3′
PMCA1bR5′-CGCCATCTTCTGCACAATT-3′5′-CAGCCATTGTTCTATTGAAAGTTC-3′5′-CAGCTGAAAGGCTTCCCGCCAAA-3′
M5′-CGCCATCTTCTGCACCATT-3′5′-CAGCCATTGCTCTATTGAAAGTTC-3′5′-CAGCTGAAAGGCTTCCCGCCAAA-3′
NCX1R5′-TCCCTATAAAACCATTGAAGGCACA-3′5′-TTTCTCATACTCCTCGTCATCGATT-3′5′-ACCTTGACTGATATTGTTTTGACTATTTCATCATTCTGGA-3′
M5′-TCCCTACAAAACTATTGAAGGCACA-3′5′-TTTCTCATACTCCTCGTCATCGATT-3′5′-ACCTTGACTGATATTGTTTTGACTATTTCATCATTCTGGA-3′