Table 1.

Regulatory region primers for SV40, BKV, and JCVa

NameDirectionSequenceReference PositionsTemperature
a The sequences of the primers used in nested PCR to amplify the regulatory regions of SV40, BKV, and JCV are shown, including the nucleotide sequences and positions from SV40-776, BKV strain PUBDUN, and JCV strain Mad-1.
RA3outer, antisense5′-GCGTGACAGCCGGCGCAGCACCA-3′nt 358-33665°C
RA4out, sense5′-GTCCATTAGCTGCAAAGATTCCTC-3′nt 5119-5142
RA1inner, antisense5′-AATGTGTGTCAGTTAGGGTGTG-3′nt 266-24562°C
RA2inner, sense5′-TCCAAAAAAGCCTCCTCACTACTT-3′nt 5195-5218
BK1-regouter, sense5′-TTCATTTTATCCTCGTCGCCC-3′nt 4996-501655°C
BK2-regouter, antisense5′-CCGTCTACACTGTCTTCACCTCTAC-3′nt 506-530
BK3-reginner, sense5′-AAAGCCTCCACACCCTTACTACTTG-3′nt 60-8455°C
BK4-reginner, antisense5′-TGTTCCAGTCCAGGTTTTACCAAC-3′nt 427-450
JC1-regouter, sense5′-GATTCCTCCCTATTCAGCACTTTG-3′nt 4985-500855°C
JC2-regouter, antisense5′-CAGGCAAAGCACTGTATCTCTGC-3′nt 5041-5062
JC3-reginner, sense5′-GCAAAAAAGGGAAAAACAAGGG-3′nt 453-47455°C
JC4-reginner, antisense5′-TGCTTTCCACTTCCCCTTGTG-3′nt 201-221