Table 2. Primers for mutation detection in PKHD1a

ExonForwardReverseSize (bp)Tm (°C)b
a Redesigned from (13).
b DHPLC column temperature, two conditions.
275′-TCACTGAGAGATTATTTTATTTGTTTTT-35-TCAGAGGGTCAGACATACTGTGA-336558, 60
32A5-CACATGCCCTACCTTCCACT-35-CTCCCACATGCAGGCTCA-344258, 60
32E5-TATGCCCCAAGTGTTTCATTATTT-35-GTGAAAGGAGCTACCAATTCATTT-358757, 58
335-GTAAAGAAGGGAGATTTGCCTGTA-35-GAATTAACCAAAGAATATCATTTCCA-326757, 59
345-GGCCTTAGGTTCTCTCTGTGG-35-AAGTTCAGGGAGGGAGAAGG-330159, 61
395′-TTTGGAGTGATGTCCTCAGTTCT-3′5′-GCAATGCCATCTATCATCAGAC-3′27959, 61
605-TTCGATATTTGTGGCTGGTG-35-GGGTTTGAAGAATTGCCAAG-329753, 55