Table 1. Influence of angiotensin II (AngII) stimulation on glucosaminoglycan composition

ECMCulture Supernatant
MediumAngIIMediumAngII
The results of three independent experiments are depicted as mean % of total glycosaminoglycans (GAG) ± SD. Percentages were calculated from the total amount of GAG, based on 35S-incorporation, that was susceptible to HNO2 treatment. Significant differences between medium and AngII in the mean % of heparan sulfate proteoglycans (HSPG) were found in the extracellular matrix (ECM) (P < 0.05) but not culture supernatant.
HSPG74.0 ± 2.7a66.3 ± 2.342.9 ± 4.445.2 ± 7.6
Non-HSPG26.0 ± 2.737.7 ± 2.357.1 ± 4.454.8 ± 7.6