Table 1. Sequences of primers and Taqman probes for real-time quantitative PCRa

GeneForward PrimerReverse PrimerProbe
a PCR primers and fluorescent probes (5′FAM-3′TAMRA) were designed using the computer program Primer Express (Applied Biosystems) and purchased from Biolegio. TRPV5, epithelial Ca2+ channel 1 (previously known as ECaC1); CaBP28, calbindin-D28K; TRPM6, putative Mg2+ channel; TRPV6, epithelial Ca2+ channel 2 (previously known as ECaC2/CaT1); CaBP9, calbindin-D9K.
TRPV55′-CTTACGGGTTGAACACCACCA-3′5′-TTGCAGAACCACAGAGCCTCTA-3′5′-TGCTTCTCAGATAGTTGTTCTTGTACTTCCTCCTTGT-3′
CaBP285′-GGAAGCTGGAGCTGACAGAGAT-3′5′-TGAACTCTTTCCCACACATTTTGAT-3′5′-ACCAGTGCAGGAAAATTTCCTTCTTAAATTCCA-3′
TRPM65′-AAAGCCATGCGAGTTATCAGC-3′5′-CTTCACAATGAAAACCTGCCC-3′5′-CCTGGTCTGAGGATGATGTTCTCAAGCC-3′
TRPV65′-ATCCGCCGCTATGCACA-3′5′-AGTTTTTCTCCTGAGTCTTTTTCCA-3′5′-TTCCAGCAACAAGATGACCTCTACTCTGA-3′
CaBP95′-CCCGAAGAAATGAAGAGCATTTT-3"5′-TTCTCCATCACCGTTCTTATCCA-3′5"-CAAAAATATGCAGCCAAGGAAGGCGA-3"