Table 1. FH intronic primers and PCR conditions

NamePrimer SequenceAnnealing TemperatureSize of Amplicon (pb)
FH20-Aaac cgt tag ttt tcc agg att taa t56°C557
    FH20-Bgtt tat tca aat caa tat gat gtt tc
H-SCR19-Accc agc cct aaa gag aaa aa55°C473
    H-SCR19-Btca cca gaa atc aca aaa ct
H-SCR18-Accc tat tac ttg tgttct g60°C406
    H-SCR18-Btca gtt gat ttg cta ctc a
H-SCR17-4cgg cta cca ata ttt ctt cag56°C460
    H-SCR17-3ctg ggc cca cac att ata taa
H-SCR16-Aacc act tac act ttg aat ga55°C408
    H-SCR16-Btag aca gac aga cac cag aa
H-SCR15-Aatg cca gaa tac aaa gtg ac60°C693
    H-SCR15-Baac aaa ata cag cca aaa tc
H-SCR14-5gtg ata att tat gaa aca gtt att g57°C404
    H-SCR14-6ctc tct tgt tta cac gaa gca c
H-SCR13-Aaaa tga gaa ata gaa tac at52°C361
    H-SCR13-Baat aac ttg gtt ggt gaa at
H-SCR12-Agaa gac tgg aaa tgt tga gg62°C441
    H-SCR12-Btgt tga tgg aga gtg gac aa
H-SCR11-Aaat aag gag ggg aag aaa gc52°C455
    H-SCR11-Batg aat aaa aga aga aaa tc
H-SCR10-Aaca tcg ttc aag aaa cag t56°C463
    H-SCR10-Bcaa cct cac ttt att gtg g
H-SCR9-Acag att tat ttt cat ttt ga50°C364
    H-SCR9-Bcca gtc ata gat tat ttt tg
H-SCR8-Atga tgt ctg ctt tgt tcc tg62°C505
    H-SCR8-Bgtc ttg atg taa tgt ctt tg
H-SCR7-Agaa aaa cca aaa act aaa ta52°C598
    H-SCR7-Bgaa aat cac agg aga aat aa
H-SCR6-3gag tgt tta tta cag taa aat ttc57°C315
    H-SCR6-4gtg ctc tcc ttt ctt cga tc
H-SCR5-Attt tat tga gtc cct att tt52°C598
    H-SCR5-Batc ctt atg aga acc aca ga
H-SCR4-Atta gca ctc tac ttt tga tt60°C370
    H-SCR4-Btac ctg atg gaa aca aca tt
H-SCR3-Acct tag aat gaa cga tgt tt50°C469
    H-SCR3-Baga aaa gaa tca gga ata aa
H-SCR2C-Attt gac tgg caa tag tga ta52°C402
    H-SCR2C-Bttt aga tag acc tgt gac tg
H-SCR2B-Attt tcc cac tct ccc ata at62°C576
    H-SCR2B-Bccc agc caa tac atc atc at
H-SCR2A-Aaaa agg aaa taa aaa tca gtt52°C500
    H-SCR2A-Bagg aga agg agg aag gaa aaa
H-SCR1-Actg tga aaa gca tca tta gc54°C390
    H-SCR1-Bata aga aaa agt cca aga gc