Table 1. Serum and glomerular IgG subclasses in C57BL/6 WT, IL-12p40−/− and IFN-γ−/− mice with EAGa

C57BL/6 WTIL-12p40−/−IFN-γ−/−
a Sera were assessed by ELISA, and glomerular deposition was assessed by direct immunofluorescence (see Materials and Methods for details). Results are expressed as the OD405 mean ± SEM and the mean ± SEM of a semiquantitative score for each animal. WT, wild type mice; IL-12p40−/−, interleukin-12p40 deficient mice; IFN-γ−/−, IFN-γ deficient mice.
b Values for normal mouse sera were 0.098 ± 0.069 for IgG1 and 0.004 ± 0.002 for IgG2a.
c P < 0.05 versus C57BL/6.
d P < 0.01 versus IL-12p40−/− mice.
IgG1
    serum (OD405)0.343 ± 0.081b0.469 ± 0.0920.503 ± 0.112
    glomerular (0–3+)2.2 ± 0.22.4 ± 0.21.5 ± 0.2c
IgG2a
    serum (OD405)0.600 ± 0.183b1.060 ± 0.0300.221 ± 0.010d
    glomerular (0–3+)0.62 ± 0.120.61 ± 0.270.10 ± 0.10