Table 1. Serum and glomerular IgG subclasses in C57BL/6 WT, IL-12p40−/− and IFN-γ−/− mice with EAGa
| C57BL/6 WT | IL-12p40−/− | IFN-γ−/− | |
|---|---|---|---|
| a Sera were assessed by ELISA, and glomerular deposition was assessed by direct immunofluorescence (see Materials and Methods for details). Results are expressed as the OD405 mean ± SEM and the mean ± SEM of a semiquantitative score for each animal. WT, wild type mice; IL-12p40−/−, interleukin-12p40 deficient mice; IFN-γ−/−, IFN-γ deficient mice. | |||
| b Values for normal mouse sera were 0.098 ± 0.069 for IgG1 and 0.004 ± 0.002 for IgG2a. | |||
| c P < 0.05 versus C57BL/6. | |||
| d P < 0.01 versus IL-12p40−/− mice. | |||
| IgG1 | |||
| serum (OD405) | 0.343 ± 0.081b | 0.469 ± 0.092 | 0.503 ± 0.112 |
| glomerular (0–3+) | 2.2 ± 0.2 | 2.4 ± 0.2 | 1.5 ± 0.2c |
| IgG2a | |||
| serum (OD405) | 0.600 ± 0.183b | 1.060 ± 0.030 | 0.221 ± 0.010d |
| glomerular (0–3+) | 0.62 ± 0.12 | 0.61 ± 0.27 | 0.10 ± 0.10 |