Table 1. PCR primer sequences and product sizesa

PrimerbOligonucleotide SequenceProduct Size (bp)Gene Bank Code
a ICAM, intracellular adhesion molecule; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
b Primer 1 is identical to the coding strand; primer 2 is complementary to the coding strand. All primers were designed such that there are intronic sequences between primer 1 and primer 2.
Class II-15′-CTCAACCACCACAACACTCTGG-3′344V01527
Class II-25′-GCCTTTTCATCCGTCACAGGAG-3′
CD40-15′-TGTGATTTGTGCCAGCCAGG-3′620M83312
CD40-25′-CCCAGGAGATGGAAGATTATCC-3′
ICAM-1-15′-TCTGTTCTTGCTGCTGTTGAGC-3′728AH001921
ICAM-1-25′-CTCTACGCAGCACAGAACATC-3′
B7.2-15′-CGGACTTGAACAACCAGACTCC-3′563L25606
B7.2-25′-GGTCACCCGAAACCTAAGAAGA-3′
GAPDH-15′-ACCACAGTCCATGCCATCAC-3′453XM_033260
GAPDH-25′-TCCACCACCCTGTTGCTGTA-3′