Table 2.

In vitro production of antibodies from different sources of lymphocytes and their specificity to pCol(28–40) or GBMa

Cell SourceIgG(μg/4 × 107 Cells)Antibody to pCol(28–40) (O.D.490nm)Antibody to GBM
ImmunofluorescencebELISAc
pCol(28–40)-immunized WKY
    ILN3.710.9770.009
    RDLN2.650.004+++0.253
pCol34A-immunized WKY
    ILN3.270.702−0.001
    RDLN0.92−0.0070.003
  • a Three independent experiments were performed, and only one experiment is shown; the other two have similar results. ILN, inguinal lymph nodes; RDLN, renal draining lymph node.

  • b For indirect immunofluorescence detection of anti-GBM antibodies, see Figure 3.

  • c IgG concentrations was adjusted to 10 ng/ml; the same concentration of normal rat IgG was used as control for background.