Table 1.

PCR primers used in this study

ConstructSense PrimerAntisense Primer
OAT4-CT-wt5′-TGAATTCGAGACCCAGGGACTTCC-3′5′-CCCTCGAGTTTCTAGAGCGAGGTAC-3′
OAT4-CT-d35′-TGAATTCGAGACCCAGGGACTTCC-3′5′-CCCTCGAGCTAACTTTCCACAGTGACGG-3′
OAT4-L550A5′-TGAATTCGAGACCCAGGGACTTCC-3′5′-CTCTCGAGCTAGGCCGAGGTACTTTCCACAG-3′
OAT4-T548A5′-TGAATTCGAGACCCAGGGACTTCC-3′5′-CCCTCGAGTTTCTAGAGCGAGGCACTTTCC-3′
NHERF1-PDZ15′-CACTCGAGATGAGCGCGGACGCAGC-3′5′-TTCTCGAGTCACCGAAGCTCGCGCTGCTC-3′
NHERF1-PDZ25′-TTCTCGAGCTTCGGCCTCGGCTCTG-3′5′-TGCTCGAGTCACTCGTCAGTTTCCCTGTC-3′
OAT4 RT-PCR5′-TCTTGCTCAGTTTCCTTGGCC-3′5′-GCTGTTGATTTCTGGCTCTCCA-3′
NHERF1 RT-PCR5′-TTCTCGAGCTTCGGCCTCGGCTCTG-3′5′-TGCTCGAGTCACTCGTCAGTTTCCCTGTC-3′