Table 2.

Specificity of PDZK1 and NHERF1 for interaction with the C-terminus of OAT4 in the yeast two-hybrid assay

C-Terminal Residues (Last 10 Amino Acids)PDZK1NHERF1
LEU2GFPLEU2GFP
OAT4-CT-wtEAVTVESTSL*++++
OAT4-CT-d3EAVTVES*
OAT4-L550AEAVTVESTSA*
OAT4-T548AEAVTVESASL*
PDZK1NHERF1
PDZ1PDZ2PDZ3PDZ4PDZ1PDZ2
LEU2+++
GFP+++
  • a The system used for the yeast two-hybrid assay included the reporter genes LEU2 and GFP, which replaced the classical lacZ gene commonly used, and allowed fast and easy detection of positive clones with long-wave UV.