Table 1.

Phenotypic characterization of female 129Sv TK−/− and WT littermates on NCa diet (0.9% wt/wt) or on LCa diet (0.01% wt/wt Ca)a

0.9% Wt/Wt Ca0.01% Wt/Wt Ca
WT (n = 19)TK−/− (n = 20)WT (n = 11)TK−/− (n = 11)
Weight (g)24.7 ± 0.923.9 ± 121.7 ± 0.621.6 ± 0.6
Food intake (g/24 h)1.92 ± 0.361.89 ± 0.351.25 ± 0.351.07 ± 0.33
Urine values
    volume (ml/24 h)1.10 ± 0.141.25 ± 0.140.90 ± 0.111.10 ± 0.11
    creatinine excretion (μmol/24 h)4.48 ± 0.334.91 ± 0.323.87 ± 0.224.00 ± 0.21
    Ca excretion (μmol/24 h)4.72 ± 0.606.85 ± 0.58b1.00 ± 0.181.49 ± 0.17
    UCa/UCr (mmol/mmol)1.09 ± 0.111.51 ± 0.18b0.25 ± 0.040.38 ± 0.04b
    UNa/UCr (mmol/mmol)36.2 ± 230.8 ± 1.937.5 ± 3.331.4 ± 3.2
    UCa/UNa (μmol/mmol)33.2 ± 7.656.6 ± 7.5b7.1 ± 1.112.2 ± 1.1b
    UMg/UCr (mmol/mmol)2.83 ± 0.183.05 ± 0.184.34 ± 0.423.78 ± 0.40
    UDPyr/UCr (nmol/mmol)9.86 ± 0.689.99 ± 2.0315.49 ± 0.63c13.64 ± 0.60c
    UcAMP/UCr (μmol/mmol)6.16 ± 0.325.62 ± 1.028.89 ± 0.66c8.69 ± 0.44c
Plasma values
    total Ca (mM)1.92 ± 0.071.87 ± 0.11.85 ± 0.11.50 ± 0.06b
    magnesium (mM)0.83 ± 0.020.80 ± 0.020.88 ± 0.020.86 ± 0.02
    protein (g/L)45.3 ± 1.945.6 ± 2.845.2 ± 0.943.8 ± 1.8
    creatinine (μM)9.7 ± 2.09.8 ± 1.69.3 ± 1.28.5 ± 1.5
    PTH (pg/ml)6.8 ± 2.212.5 ± 4.226.7 ± 16.329.9 ± 14.8
    1,25-dihydroxyvitamin D (pmol/L)64 ± 1276 ± 15113 ± 1372 ± 8b
  • a TK−/−, tissue kallikrein–deficient; WT, wild-type; UCa, urinary calcium; UCr, urinary creatinine; UNa, urinary sodium; UMg, urinary magnesium; UcAMP, urinary cAMP; PTH, parathyroid hormone.

  • b P < 0.05 versus WT on same diet.

  • c P < 0.05 versus NCa diet.