Table 2.

Phenotypic characterization of female C57Bl6/J TK−/− and WT littermates on NCa diet (0.9% wt/wt Ca) or on LCa diet (0.01% wt/wt Ca)a

0.9% wt/wt Ca0.01% wt/wt Ca
WT (n = 26)TK−/− (n = 23)WT (n = 10)TK−/− (n = 9)
Weight (g)21.9 ± 0.422.1 ± 0.420.3 ± 0.421.9 ± 0.5b
Food intake (g/24 h)3.77 ± 0.224.11 ± 0.123.48 ± 0.154.33 ± 0.30b
Urine values
    volume (ml/24 h)1.04 ± 0.091.52 ± 0.260.82 ± 0.151.20 ± 0.28
    creatinine excretion (μmol/24 h)2.89 ± 0.193.39 ± 0.292.87 ± 0.373.24 ± 0.34
    Ca excretion (μmol/24 h)1.36 ± 0.172.16 ± 0.35b0.75 ± 0.151.41 ± 0.25b
    UCa/UCr (mmol/mmol)0.46 ± 0.040.61 ± 0.08c0.24 ± 0.030.49 ± 0.1b
    Na excretion (μmol/24 h)231 ± 14275 ± 22230 ± 33252 ± 34
    UNa/UCr (mmol/mmol)84.2 ± 4.483.5 ± 4.778.9 ± 3.477.2 ± 5.1
    UCa/UNa (μmol/mmol)6.2 ± 0.98.1 ± 1.13.1 ± 0.36.6 ± 1.4b
    UPi/UCr (mmol/mmol)61.7 ± 3.566.7 ± 5.980.6 ± 4.982.9 ± 7.0
    UcAMP/UCr (μmol/mmol)5.13 ± 0.435.40 ± 0.286.21 ± 0.36d7.28 ± 0.74d
    UDPyr/UCr (nmol/mmol)14.87 ± 1.0914.94 ± 0.5919.18 ± 0.80d19.62 ± 1.28d
Plasma values
    total Ca (mM)1.94 ± 0.041.90 ± 0.071.98 ± 0.071.98 ± 0.04
    1,25-dihydroxy vitamin D (pmol/L)210 ± 27262 ± 38563 ± 65517 ± 68
    protein (g/L)37.0 ± 0.635.6 ± 0.636.1 ± 0.535.8 ± 0.6
    creatinine (μM)14.4 ± 1.215.5 ± 1.59.9 ± 0.911.8 ± 1.7
    PTH (pg/ml)26.0 ± 7.413.8 ± 1.435.6 ± 1.431.2 ± 11.7
    Pi (mM)3.29 ± 0.283.29 ± 0.113.44 ± 0.123.02 ± 0.13b
  • a Pi, inorganic phosphate.

  • b P < 0.05 versus WT.

  • c P = 0.069 versus WT.

  • d P < 0.01 versus NCa diet.