Table 4.

Phenotypic characterization of C57Bl/6 B2−/− and WT littermates and B2−/− mice that were treated with a B1 receptor antagonist on NCa diet (0.9% wt/wt Ca)

0.9% wt/wt Ca
WT (n = 10)B2−/− (n = 10)B2−/− (n = 12)
TreatmentVehicleVehicleB1 antagonist SSR240612
Weight (g)22.1 ± 0.520.9 ± 0.623.0 ± 0.5
Food (g/24 h)2.99 ± 0.233.44 ± 0.313.73 ± 0.25
Urine values
    volume (ml/24 h)0.94 ± 0.170.90 ± 0.181.26 ± 0.13
    creatinine excretion (μmol/24 h)2.68 ± 0.272.51 ± 0.273.20 ± 0.24
    Ca excretion (μmol/24 h)1.46 ± 0.391.48 ± 0.371.75 ± 0.35
    UCa/UCr (mmol/mmol)0.50 ± 0.080.53 ± 0.110.53 ± 0.08
    UNa/UCr (mmol/mmol)72.0 ± 8.986.8 ± 8.792.8 ± 5.3
    UCa/UNa (μmol/mmol)7.21 ± 0.936.63 ± 0.807.12 ± 0.50
Plasma values
    total plasma Ca (mM)1.82 ± 0.041.78 ± 0.051.86 ± 0.04
    1,25-dihydroxyvitamin D (pmol/L)217 ± 33265 ± 41281 ± 31