Table 5.

Effect of Ca intake on the mRNA expression of several Ca2+ transport proteins in the kidney of 129Sv TK−/− and WT micea

0.9% wt/wt Ca0.01% wt/wt Ca
WTTK −/−WTTK −/−
TRPV5 (×103)71 ± 1068 ± 762 ± 786 ± 9
Calbindin-D28k3.17 ± 0.223.08 ± 0.302.62 ± 0.282.36 ± 0.15
Calbindin-D9k13.20 ± 2.0511.58 ± 1.4121.65 ± 2.52b20.58 ± 1.90c
TRPV6 (×103)16.9 ± 1.313.4 ± 1.015.1 ± 0.916.7 ± 1.6
NCX1 (×103)3.7 ± 0.33.8 ± 0.34.8 ± 0.83.6 ± 0.2
PMCA1b (×106)60 ± 450 ± 355 ± 148 ± 2
  • a Renal mRNA levels of TRPV5, Calbindin-D28k, and Calbindin-D9k genes are assessed by quantitative real-time PCR and expressed as a ratio of the copy number of the target gene and the copy number of hypoxanthine-guanine phosphoribosyl transferase gene (n = 7 per group).

  • b P < 0.05 versus NCa diet.

  • c P < 0.01 versus NCa diet.