Table 2.

Supplemental movie available online at http://www.jasn.org

Supplemental Movie 1. Mating behavior of wild-type C. elegans male (courtesy of Luis Rene Garcia, Texas A&M University). The male responds to hermaphrodite contact by putting his tail flush on her body; he begins backing until he encounters her head or tail, where he turns via a sharp ventral coil. He continues backing until he reaches the vulva, where he stops, inserts his spicules, and ejaculates into the hermaphrodite uterus. Completion of all sub-behaviors is not mandatory for successful copulation. For example, if the initial contact is on the ventral side of the hermaphrodite, then the male may immediately locate the vulva, insert his spicules, and ejaculate without evoking turning behavior.
Supplemental Movie 2. Mating behavior of pkd-2 mutant male. When the male tail contacts the hermaphrodite body, pkd-2 mutants fail to respond and continue moving forward. Occasionally, pkd-2 mutants will respond. pkd-2 mutants back and turn normally. However, the pkd-2 mutant fails to sense and stop at the hermaphrodite’s vulva and is categorized as location-of-vulva (Lov)–defective.