Table 3.

Renal injury in experimental mice at age 8 mo

db/+ (n = 10)db/db (n = 10)ICAM-1−/− db/db (n = 10)
Renal function
    UAER, μg/18 h7.8 ± 1.189.3 ± 22.8b19.9 ± 8.5b,f
    plasma creatinine, μmol/L23.4 ± 1.035.0 ± 2.9b32 ± 2.4a
    CrCl, μl/min156.9 ± 26.055.3 ± 7.4c75.4 ± 10.6b
Glomerular damage
    volume, μm3 × 1042.95 ± 0.148.67 ± 2.2a4.77 ± 0.45
    cellularity, cells/gcs34.5 ± 0.856.7 ± 2.9c42.7 ± 4.2a,f
    TGF-β1, % area4.3 ± 0.613.9 ± 1.7c8.5 ± 3.8d
    collagen IV, % area17.5 ± 0.421.5 ± 1.0c17.5 ± 0.5c,f
Tubular damage
    atrophic tubules, %0.05 ± 0.019.7 ± 1.2c4.2 ± 1.2a,e
Tubulointerstitial fibrosis
    α-SMA, % area1.3 ± 0.24.4 ± 0.7c1.8 ± 0.1f
    collagen IV, % area17.2 ± 0.525.3 ± 0.5c20.2 ± 0.3c,f

  • Data = mean ± SE.

  • ↵a P < 0.05,

  • ↵b P < 0.01,

  • ↵c P < 0.001 versus db/+;

  • ↵d P < 0.05,

  • ↵e P < 0.01,

  • ↵f P < 0.001 for db/db ICAM-1−/− versus db/db.

  • CrCl indicates creatinine clearance; SMA, smooth muscle actin; UAER, urine albumin excretion rate.