Table 1.

Primers used in PCR for generation of FGF23 promoter constructsa

PairSense Primer/Antisense PrimerLocation
15′-GCGGTACCTATGACCATATATCAAGACACTTGCC-3′5′-GCAAGCTTTGCACAGCACTGAGTGGCTAATGC-3′−600/−1
25′-TGGTACCGCATGCACAGAGCAATTTCTGC-3′5′-GCAAGCTTTGCACAGCACTGAGTGGCTAATGC-3′−1000/−1
35′-GGGTACCATCCATGTGCTTTACACGATGG-3′5′-GCAAGCTTTGCACAGCACTGAGTGGCTAATGC-3′−1300/−1
45′-TGGTACCTAGCTGTGGCTATGATTCATCC-3′5′-GCAAGCTTTGCACAGCACTGAGTGGCTAATGC-3′−2000/−1
55′-GAGGTACCTCATCTATGGAGTAGACTCC-3′5′-GCAAGCTTTGCACAGCACTGAGTGGCTAATGC-3′−3550/−1
  • a Underlines indicate restriction enzyme sites.