Table 2.

Primer sequences for PCR construct probes used in ISHa

TargetSequence (5′-3′)Orientation
γ-ENaCGCCCGTCACAAACATCTACAATGCTGCSense
γ-ENaCCTTGGCCAGGTCAGTCTTGTTGAGCAntisense
NCCGCATTCATTCCTCAAGCAGGAAGGTAGSense
NCCGGTGTGCCATACTCCTGCAGTAGGAntisense
NKCC2AAGATGTCGGTCAGCATCCCTTCCSense
NKCC2CCATCATTGAATCGCTCTCCTTAAGAAGAAntisense
WNK4GAACGAGAGGTTCACCATCCAGGSense
WNK4CATCTGAGGCGTAGCTATCCCCAGAntisense
  • a Primer sequences for WNK1 are detailed in reference (7). For additional information, contact the corresponding author. γ-ENaC, γ subunit of epithelial Na channel; NCC, thiazide-sensitive Na+Cl co-transporter; NKCC2, Na+K+2Cl co-transporter type 2.