Table 1.

Primer set for sodium transporter analysis by real-time PCRa

mRNAPrimer
ROMK
    senseTCA GAC CAA TAT AAA CTT
    reverseTGG GAA AGA GTT TCT GCCG
NKCC2
    senseATC TTT GCT TTT GCC AAT GC
    reverseATG TCA TTG GTT GGA TCC AC
ENaC-α
    senseTAC GCG ACA ACA ATC CCC AAG TGG
    reverseATG GAA GAC ATC CAG AGA TTG GAG
ENaC-β
    senseCAT AAT CCT AGC TTG CCT GTT TGG A
    reverseCAG TTG CCA TAA TCA GGG TAG AAG A
ENaC-γ
    senseTGC AAG CAA TCC TGT AGC TTT AAG
    reverseGAA GCC TCA GAT GGC CAC TGT
Na+/K+-ATPase α1
    senseCTC CAG CAA CAG GAC CCG GCG
    reverseGAT CTC AGC GGC CCT TGC AGG
NHE3
    senseGTA GTT GTG TGC CAG GTT CTC
    reverseGGA ACA GAG GCG GAG GAG CAT
β-actin
    senseAGA CCT TCA ACA CCC CAG CC
    reverseACA GCA CTG TGT TGG CAT AG
  • a ENaC, epithelial sodium channel; NHE3, Na+/H+-exchanger, NKCC2, Na+-K+-2Cl co-transporter; ROMK, renal outer medullary potassium channel.