Table 2.

Effect of LPS (10 mg/kg intraperitoneally) without or with dexamethasone (10 mg/kg intraperitoneally) and IL-1β (1 μg/g intraperitoneally) on hemodynamic, blood, and renal parametersa

ParameterControlLPSLPS + DexamethasoneIL-1β
6 h12 h24 h6 h12 h24 h12 h
Weight (g)20.0 ± 0.620.1 ± 0.219.4 ± 0.419.6 ± 0.219.9 ± 0.319.7 ± 0.219.7 ± 0.119.9 ± 0.3
MAP (mmHg)74 ± 161 ± 2b50 ± 2b47 ± 3b64 ± 3b62 ± 2b,c60 ± 2b,c54 ± 3b
Heart rate (beats/min)486 ± 11527 ± 7b587 ± 12b625 ± 13b507 ± 10576 ± 12b610 ± 12b589 ± 12b
Plasma Na+ (mmol/L)144.5 ± 0.7145.1 ± 0.7150.5 ± 1.0b151.7 ± 0.9b145.5 ± 0.9149.9 ± 0.7b150.7 ± 1.3b150.1 ± 1.1b
Hemoglobin (g/dl)13.1 ± 0.513.1 ± 0.312.9 ± 0.213.1 ± 0.213.4 ± 0.413.3 ± 0.313.1 ± 0.213.2 ± 0.4
Hematocrit (%)37.1 ± 0.836.1 ± 0.536.3 ± 1.336.2 ± 1.236.3 ± 1.037.3 ± 1.136.1 ± 1.136.6 ± 1.3
Urine flow (μl/min)0.62 ± 0.030.51 ± 0.03b0.32 ± 0.02b0.30 ± 0.03b0.58 ± 0.040.44 ± 0.03b,c0.44 ± 0.02b,c0.35 ± 0.06b
GFR (μl/min per g body wt)10.3 ± 0.47.1 ± 0.2b2.7 ± 0.2b1.5 ± 0.1b8.8 ± 0.2b,c3.8 ± 0.3b,c3.4 ± 0.6b,c3.0 ± 0.6b
Urinary Na+ excretion (nmol/min per g body wt)12.1 ± 0.510.1 ± 0.68.5 ± 1.0b8.0 ± 1.1b11.0 ± 0.78.6 ± 0.8b10.2 ± 0.6b9.2 ± 0.6b
Fractional Na+ excretion (%)0.88 ± 0.110.93 ± 0.152.10 ± 0.20b3.76 ± 0.27b0.86 ± 0.171.39 ± 0.14b,c2.01 ± 0.21b,c1.96 ± 0.14b
Tubular Na+ reabsorption (μmol/min)27.6 ± 0.521.3 ± 0.5b8.1 ± 0.5b4.2 ± 0.6b25.2 ± 0.5b,c12.2 ± 0.5b,c10.1 ± 0.6b,c9.3 ± 0.7b
  • a Data are means ± SEM, n = 6. MAP, mean arterial pressure.

  • b P < 0.05 versus control.

  • c P < 0.05 versus respective LPS.