Table 1.

Control conditionsa

GenotypeAe1+/+Ae1+/−Ae1−/−
Blood
    pH7.27 ± 0.027.30 ± 0.027.12 ± 0.02b
    HCO322.0 ± 0.822.2 ± 0.317.3 ± 0.5b
    Pco248.8 ± 1.744.9 ± 1.055.8 ± 2.1
    K+3.7 ± 0.13.5 ± 0.04.4 ± 0.1c
    Na+147.3 ± 1.1145.2 ± 0.8149.1 ± 1.5
    Cl113.3 ± 1.1112.2 ± 1.1116.7 ± 1.2c
    Pi1.6 ± 0.11.6 ± 0.12.4 ± 0.3c
    anion gap12.0 ± 0.810.8 ± 0.215.1 ± 1.1
    osmolarity315.1 ± 2.6310.2 ± 1.5336.0 ± 9.1c
    creatinine0.07 ± 0.01ND0.11 ± 0.02
    urea6.4 ± 1.6ND26.8 ± 3.8b
Urine
    pH6.48 ± 0.166.38 ± 0.136.77 ± 0.12
    osmolarity2519.8 ± 176.32336 ± 279.11085.1 ± 103.1b
    creatinine68.9 ± 3.866.2 ± 6.627.1 ± 3.4b
    HCO3/creatinine0.2 ± 0.10.1 ± 0.00.5 ± 0.2
    K+/creatinine7.4 ± 1.02.9 ± 0.4c6.9 ± 1.1
    Na+/creatinine3.5 ± 0.51.2 ± 0.2c3.3 ± 0.5
    Cl/creatinine3.8 ± 0.33.7 ± 0.64.2 ± 0.3
    Pi/creatinine0.7 ± 0.10.7 ± 0.10.5 ± 0.1
    NH4+/creatinine1.2 ± 0.21.0 ± 0.21.4 ± 0.2
    Ca2+/creatinine0.05 ± 0.010.05 ± 0.010.11 ± 0.02c
    Mg2+/creatinine0.44 ± 0.090.19 ± 0.040.66 ± 0.19
    citrate/creatinine0.71 ± 0.09ND0.25 ± 0.04b
    creatinine clearance1.03 ± 0.17ND1.11 ± 0.22
    FE Na+ (%)0.26 ± 0.05ND0.68 ± 0.15c
    FE K+ (%)21.25 ± 4.11ND10.31 ± 2.23
    FE Cl (%)0.31 ± 0.08ND0.31 ± 0.05
    FE Ca2+ (%)0.76 ± 0.09ND0.58 ± 0.08
    FE Mg2+ (%)5.62 ± 2.76ND6.88 ± 1.16
    FE Pi (%)2.09 ± 0.42ND1.81 ± 0.44
    urinary anion gap177.8 ± 89.321.4 ± 36.336.8 ± 24.2
    titratable acids38.7 ± 4.628.0 ± 6.15.5 ± 6.6b
    NEA120.0 ± 10.294.1 ± 14.649.1 ± 12.4b
    NEA/creatinine1.9 ± 0.21.4 ± 0.11.6 ± 0.4
  • a Summary of basal blood and urine data. Urine was collected during 24 h in metabolic cages, and blood was sampled at the end of the collection period. Concentrations of electrolytes, titratable acids, and urea are expressed in units of mmol/L, creatinine in mg/dl, Pco2 in mmHg, osmolarity in mOsmol/L, creatinine clearance in ml/min; n = 8 to 10 animals per genotype. ND, not determined; NEA, net acid excretion.

  • b P < 0.001 versus wild-type.

  • c P < 0.05 versus wild-type.