Table 2.

24-h NH4Cl loadinga

GenotypeAe1+/+Ae1+/−Ae1−/−
Blood
    pH7.21 ± 0.017.20 ± 0.016.82 ± 0.05b
    HCO320.6 ± 0.718.8 ± 0.77.1 ± 0.9b
    Pco253.3 ± 1.849.6 ± 1.544.4 ± 3.1c
    K+3.7 ± 0.24.0 ± 0.14.9 ± 0.3b
    Na+147.9 ± 1.0148.1 ± 1.0156.4 ± 2.8c
    Cl117.0 ± 1.8119.9 ± 2.0133.0 ± 4.3b
    Pi2.0 ± 0.22.1 ± 0.23.4 ± 0.1b
    anion gap10.1 ± 1.89.5 ± 2.316.3 ± 2.4c
    osmolarity304.1 ± 1.2302.4 ± 1.5346.8 ± 3.4b
    creatinine0.08 ± 0.020.10 ± 0.020.15 ± 0.02c
    urea10.8 ± 1.6ND29.6 ± 5.3b
Urine
    pH6.07 ± 0.066.10 ± 0.106.47 ± 0.12c
    osmolarity3302.5 ± 178.42388 ± 97.11176.5 ± 69.7b
    creatinine83.1 ± 5.886.1 ± 13.133.2 ± 3.3b
    HCO3/creatinine0.0 ± 0.00.1 ± 0.00.1 ± 0.0
    K+/creatinine3.5 ± 0.63.1 ± 0.75.2 ± 1.0
    Na+/creatinine1.8 ± 0.41.4 ± 0.33.5 ± 0.7
    Cl/creatinine7.6 ± 0.57.3 ± 1.06.7 ± 1.0
    Pi/creatinine0.6 ± 0.10.5 ± 0.10.8 ± 0.2
    NH4+/creatinine2.1 ± 0.22.2 ± 0.32.2 ± 0.3
    Ca2+/creatinine0.04 ± 0.010.05 ± 0.020.06 ± 0.01
    Mg2+/creatinine0.19 ± 0.040.20 ± 0.080.26 ± 0.07
    creatinine clearance1.46 ± 0.400.80 ± 0.310.47 ± 0.06b
    FE Na+ (%)0.10 ± 0.030.09 ± 0.050.34 ± 0.08c
    FE K+ (%)6.78 ± 2.063.99 ± 1.0516.72 ± 4.06
    FE Cl (%)0.44 ± 0.100.58 ± 0.220.76 ± 0.15
    FE Ca2+ (%)0.15 ± 0.060.24 ± 0.110.31 ± 0.07
    FE Mg2+ (%)4.42 ± 1.692.41 ± 1.783.80 ± 1.08
    FE Pi (%)1.98 ± 0.433.02 ± 1.013.52 ± 0.62
    urinary anion gap−202.3 ± 69.7−223.2 ± 57.680.6 ± 61.8c
    titratable acids37.8 ± 3.220.0 ± 4.716.1 ± 4.4c
    NEA205.7 ± 9.5176.6 ± 10.285.2 ± 5.2b
    NEA/Crea2.6 ± 0.22.9 ± 0.52.7 ± 0.3
  • a Summary of blood and urine data after 24 h of oral NH4Cl loading. Urine was collected during 24 h in metabolic cages, and blood was sampled at the end of the collection period. Concentrations of electrolytes, titratable acids, and urea are expressed in units of mmol/L, creatinine in mg/dl, Pco2 in mmHg, osmolarity in mOsmol/L, creatinine clearance in ml/min; n = 8 to 10 animals per genotype.

  • b P < 0.001 versus wild-type.

  • c P < 0.05 versus wild-type.