Table 1.

Serum factors and lymphoproliferation in 24-wk-old MLR lpr/lpr micea

ParameterSalineIRS 661IRS 954
Serum IL-6 (pg/ml)90.3 ± 31.358.1 ± 16.826.9 ± 3.1b
    TNF (pg/ml)23.9 ± 4.04.4 ± 2.3b5.8 ± 4.4b
    IL-12p40 (ng/ml)5.3 ± 1.13.4 ± 0.7b4.6 ± 0.7
    IFN-α (pg/ml)35.4 ± 8.330.6 ± 7.537.5 ± 7.9
    IgG1 (mg/ml)38.4 ± 4.729.4 ± 4.834.4 ± 4.0
    IgG2a (mg/ml)12.0 ± 1.39.1 ± 1.712.9 ± 1.1
    IgG2b (mg/ml)9.2 ± 2.16.1 ± 1.15.8 ± 1.4
    IgG3 (mg/ml)3.2 ± 0.23.3 ± 0.32.9 ± 0.2
Anti-dsDNA IgG1 (μg/ml)15.0 ± 6.314.7 ± 6.234.2 ± 12.7
    IgG2a (μg/ml)38.0 ± 7.118.9 ± 2.7b56.8 ± 12.9c
    IgG2b (μg/ml)46.3 ± 9.518.2 ± 2.3b34.8 ± 7.2c
    IgG3 (μg/ml)27.7 ± 3.721.8 ± 2.124.1 ± 2.2
Anti-Sm IgG (μg/ml)8.8 ± 3.44.4 ± 1.1b10.2 ± 2.8c
Rheumatoid factor (OD [450 nm])0.88 ± 0.140.86 ± 0.130.81 ± 0.13
Lymph node weight (mg/g body wt)16.7 ± 1.78.5 ± 1.6b9.1 ± 0.6b
Spleen weight (mg/g body wt)8.9 ± 0.86.7 ± 0.5b6.1 ± 0.4b
    CD4+ cells (% spleen)18.1 ± 3.417.6 ± 4.819.4 ± 4.3
    CD8+ cells (% spleen)8.7 ± 1.79.7 ± 2.79.0 ± 2.0
    CD4/CD8 (% spleen)62.3 ± 1.451.7 ± 3.4b57.5 ± 1.5b
  • a n = 10 per group; data are means ± SEM. IRS, immunoregulatory sequence.

  • b P < 0.05 IRS 661 or IRS 954 versus saline.

  • c P < 0.05 IRS 954 versus IRS 661 by unpaired t test.