Table 1.

Frequency of immunogold-positive junctions of nephrotic foot processesa

ParameterFrequency of Labeled Junctions (%)bGold Particles Associated with Cell–Cell Junctions (%)c
SDTJ-Like Junctions
CARND35.6 ± 10.497.5 ± 5.0
Podocin68.8 ± 8.666.4 ± 16.692.7 ± 8.2
  • a Ultrathin sections of rat glomeruli at day 4 after puromycin aminonucleoside injection were double labeled for podocin and coxsackievirus and adenovirus receptor (CAR) by gold particles of 5 and 15 nm, respectively, as described in the Concise Methods section. ND, not detectable; SD, slit diaphragm; TJ, tight junction.

  • b Frequency of podocin- or CAR-labeled junctions versus nonlabeled ones is shown as a percentage in total of 50 to 100 of SD or TJ-like structures (mean ± SEM from six independent experiments). The cell–cell junctions that had a discrete gap (>20 nm) are designated as SD, whereas those with no visible intercellular space are called TJ-like structures.

  • c Frequency of immunogold particles accumulating at cell–cell junctions (either SD or TJ-like structures) versus at the reminder locations is shown as a percentage of total number of foot process–associated gold marks (n = 100 to 200). The criteria that discriminate junctional labelings from those locating anywhere else in the foot process cytoplasm are according to Wernerson et al. (Nephrol Dial Transplant 18: 70–76, 2003). Data are means ± SEM from six independent experiments.