Table 1.

Plasma and urine osmolality (mOsm/kg H2O); free water clearance (μ l/min); and plasma concentrations of potassium (mmol/L), aldosterone (nmol/L), and corticosterone (nmol/l) in four cohorts of 11βHSD2−/− and C57BL/6J micea

ParameterAge (d)
356080120
POsm
    C57BL/6J305 ± 3304 ± 3312 ± 2306 ± 7
    11βHSD2−/−315 ± 3b311 ± 2b313 ± 4295 ± 2
UOsm
    C57BL/6J1801 ± 1161630 ± 2402007 ± 1701572 ± 75
    11βHSD2−/−779 ± 67c647 ± 45c604 ± 99c609 ± 40c
FWC
    C57BL/6J−5.9 ± 0.9−5.8 ± 0.7−3.6 ± 0.6−4.1 ± 0.5
    11βHSD2−/−−3.1 ± 0.7b−3.2 ± 0.6b−2.2 ± 0.6b−1.6 ± 0.3c
PAldo
    C57BL/6J1.57 ± 0.241.60 ± 0.021.31 ± 0.181.07 ± 0.09
    11βHSD2−/−0.63 ± 0.12c0.44 ± 0.07c0.69 ± 0.07c0.45 ± 0.05c
PCort
    C57BL/6J374 ± 90323 ± 20338 ± 80NA
    11βHSD2−/−286 ± 58289 ± 35242 ± 22NA
  • a Data are means ± SE. Statistical comparisons were made using ANOVA with least significant difference post hoc test. ANOVA indicated significant effect of genotype in all four variables. There was no effect of age per se for any variable, but there was a significant interaction between age and genotype for UOsm (P < 0.01), FWC (P < 0.01), and PK (P < 0.01). FWC, free water clearance; PAldo, plasma concentration of aldosterone; PCort, plasma concentration of corticosterone; PK, plasma concentration of potassium; POsm, plasma osmolality; UOsm, urine osmolality

  • b P < 0.05.

  • c P < 0.01.