Table 2.

Myography data from 11βHSD2−/− and aged-matched C57BL/6J micea

ParameterAge
35 (n = 6)60 (n = 6)80 (n = 6)120 (n = 7)
Maximum response (EMax, mN/mm or % relaxation)
    NA
        C57BL/6J1.84 ± 0.243.08 ± 0.352.34 ± 0.422.78 ± 0.41
        11βHSD2−/−1.42 ± 0.202.53 ± 0.522.72 ± 0.282.23 ± 0.20
    KCl
        C57BL/6J1.65 ± 0.072.09 ± 0.102.22 ± 0.173.25 ± 0.34
        11βHSD2−/−1.69 ± 0.232.56 ± 0.152.60 ± 0.072.43 ± 0.32
    ACh
        C57BL/6J74.0 ± 7.478.4 ± 2.274.0 ± 4.485.1 ± 3.5
        11βHSD2−/−79.3 ± 6.763.5 ± 4.6b78.8 ± 3.884.3 ± 6.1
    SNP
        C57BL/6J101.8 ± 4.4102.6 ± 1.7101.9 ± 1.6103.0 ± 2.1
        11βHSD2−/−102.7 ± 3.2100.3 ± 3.299.1 ± 1.1101.3 ± 1.3
Sensitivity (pD2; logIC50)
    NA
        C57BL/6J7.75 ± 0.128.07 ± 0.137.91 ± 0.197.70 ± 0.18
        11βHSD2−/−7.64 ± 0.187.82 ± 0.078.00 ± 0.037.54 ± 0.07
    KCl
        C57BL/6J1.79 ± 0.161.76 ± 0.191.79 ± 0.141.64 ± 0.08
        11βHSD2−/−1.67 ± 0.081.60 ± 0.181.91 ± 0.271.45 ± 0.07
    ACh
        C57BL/6J7.03 ± 0.197.46 ± 0.147.53 ± 0.117.37 ± 0.14
        11βHSD2−/−6.84 ± 0.247.25 ± 0.147.19 ± 0.157.30 ± 0.15
    SNP
        C57BL/6J7.78 ± 0.128.19 ± 0.178.05 ± 0.177.95 ± 0.12
        11βHSD2−/−7.75 ± 0.097.86 ± 0.137.67 ± 0.118.04 ± 0.12
  • a Data are means ± SEM. Comparisons were made using t test. ACh, acetylcholine; KCl potassium chloride; NA, noradrenaline; SNP, sodium nitroprusside.

  • b P = 0.0134.