Table 1.

Effect of anti-integrin antibodies on cell height and cross-sectional areaa

ParameternHeight (μ ± SEM)PArea (μ ± SEM)P
Integrin blockade of HD cells (ANOVA)
    HD69.190 ± 0.070206.17 ± 5.38
        + anti-αvβ165.248 ± 0.070<0.001479.50 ± 6.23<0.001
        + anti-α169.140 ± 0.120>0.05 (NS)211.83 ± 8.48>0.05 (NS)
        + anti-α667.795 ± 0.090<0.001313.95 ± 5.57<0.001
LD with activating anti–integrin β1 antibodies (unpaired t test)
    LD65.230 ± 0.050989.08 ± 6.30
        + anti-β168.850 ± 0.040<0.0001b407.15 ± 13.00<0.0001b
Integrin blockade of LD cells seeded on polymerized hensin (ANOVA)
    LD55.110 ± 0.0401011.00 ± 7.70
    LD on hensin59.320 ± 0.080470.23 ± 7.80
        + anti-αvβ159.210 ± 0.020>0.05 (NS)c472.65 ± 5.40>0.05 (NS)c
        + anti-α159.270 ± 0.020>0.05 (NS)c458.10 ± 5.00>0.05 (NS)c
        + anti-α655.490 ± 0.050<0.001c2139.30 ± 14.00<0.001c
  • a Results obtained from the measurements of cell height and surface area from confocal images of phalloidin/Sytox green–stained cells as described in the Concise Methods section. HD, high-density; LD, low-density; n, number of independent experiments.

  • b Two-tailed.

  • c Versus LD on hensin.