Table 1.

Urine protein quantification by dipstick analysis, protein concentration, and protein-creatinine ratio over time in wild-type, CRE(−);NOTCH-IC, and CRE(+);NOTCH-IC mice

ParameterUrine Protein at Postnatal Age
P0P7P14P21P42
Wild type
    median urine dip test (lower quartile, upper quartile)Trace (tr, tr)Trace (tr, tr)Trace (tr, 0.3)Trace (tr, 0.3)0.3 g/L (0.3, 0.3)
(n = 3)(n= 5)(n = 7)(n = 4)(n = 4)
    protein (mg/L)118 ± 5746 ± 5117 ± 43213 ± 16247 ± 70
    protein:creatinine (mg/mmol)97 ± 2834 ± 322 ± 331 ± 461 ± 13
(n = 3)(n = 5)(n = 7)(n = 4)(n = 4)
CRE(−);NOTCH-IC
    median urine dip test (lower quartile, upper quartile)Trace (tr, tr)Trace (tr, 0.3)Trace (tr, 0.3)Trace (tr, tr)Trace (tr, 0.3)
(n = 10)(n = 6)(n = 13)(n= 6)(n = 5)
    protein (mg/L)24 ± 952 ± 7177 ± 43123 ± 28367 ± 98
    protein:creatinine (mg/mmol)54 ± 2818 ± 3a67 ± 13a64 ± 24108 ± 17
(n = 2)(n = 3)(n = 5)(n = 4)(n = 5)
CRE(+);NOTCH-IC
    median urine dip test (lower quartile, upper quartile)Trace (tr, tr)Trace (tr, 0.3)3.0 (3.0, >20)>20 g/L (>20, >20)>20 g/L (>20, >20)
(n = 12)(n = 5)(n = 8)(n = 8)(n = 4)
    protein (mg/L)17 ± 6281 ± 1463535 ± 5133273 ± 4523179 ± 833
    protein:creatinine (mg/mmol)31 ± 17138 ± 64217 ± 51a,b2011 ± 464a,b3359 ± 1567
(n = 4)(n = 4)(n = 4)(n = 5)(n = 4)
  • a P < 0.05 versus wild-type.

  • b P < 0.05 versus CRE(−);NOTCH-IC.