Table 5.

Human urinary exosome phosphopeptidesa

Ref SeqProtein Name, SequenceSiteGeneNovel SiteMSnMotifGO Function
NP_061123G protein–coupled receptor family C, group 5, member C isoform bGPRC5CMetabotropic glutamate, GABA-B–like receptor activity Protein binding Receptor activity
R.AEDMYSAQSHQAA(T*)PPKDGK.NT435YesMS2, MS3Proline-directed
K.VP(S*)EGAYDIILPR.AS395YesMS2, MS3Phosphoserine/threonine binding group
R.AEDM(Y*)SAQSHQAATPPKDGK.NY426YesMS2Tyrosine kinase
NP_001035149Secreted phosphoprotein 1 isoform cSPP1Cytokine activity Growth factor activity Integrin binding Protein binding
K.AIPVAQDLNAPSDWD(S*)R.GS192NoMS2Miscellaneous
R.GKD(S*)YETSQLDDQSAETHSHK.QS197MS2, MS3Basophilic
R.GKDSYETSQLDDQ(S*)AETHSHK.QS207MS2, MS3Acidophilic
NP_057319G protein–coupled receptor, family C, group 5, member B precursorGPRC5BMetabotropic glutamate, GABA-B–like receptor activity Receptor activity Sevenless binding
R.SNVYQPTEMAVVLNGG(T*)IPTAPPSHTGR.HT389YesMS2Basophilic
NP_000477Aquaporin 2AQP2Transporter activity Water channel activity
R.RQ(S*)VELHSPQSLPR.GS256NoMS2, MS3Basophilic
NP_004860Vacuolar protein sorting factor 4BVPS4BATP binding ATPase activity, Coupled nucleotide binding Protein binding
K.EGQPSPADEKGND(S*)DGEGESDDPEKKK.LS102YesMS2Acidophilic
NP_054762Chromatin modifying protein 2BCHMP2BNot classified
K.ATI(S*)DEEIER.QS199NoMS2, MS3 (unfiltered)Acidophilic
NP_687033Proteasome α 3 subunit isoform 2PSAM3Protein binding Threonine endopeptidase activity
K.ESLKEEDE(S*)DDDNMS243NoMS2, MS3Acidophilic
NP_036382Related RAS viral (r-ras) oncogene homolog 2RRAS2GTP binding Nucleotide binding Protein binding
R.KFQEQECPP(S*)PEPTRK.ES186YesMS2, MS3Proline-directed
NP_031381Heat-shock 90-kD protein 1, βHSP90AB1ATP binding Nitric-oxide synthase regulator activity Nucleotide binding TPR domain binding Unfolded protein binding
K.IEDVG(S*)DEEDDSGKDKK.KS255NoMS2Acidophilic
NP_612433Kinesin family member 12KIF12ATP binding Microtubule motor activity Nucleotide binding
R.VTTRPQAPK(S*)PVAK.QS236YesMS2, MS3Proline-directed
NP_079119Cytochrome b reductase 1CYBRD1Ferric-chelate reductase activity
R.NLALDEAGQRS(T*)M.T285YesMS2Basophilic
NP_001037857Mucin 1 isoform 7 precursorMUC1NF-κB binding protein heterodimerization activity
R.DTYHPMSEYPTYH(T*)HGR.YT118YesMS2, MS3 (unfiltered)AcidophilicProtein homodimerization activity RNA binding Tat protein binding Unfolded protein binding
NP_000330Solute carrier family 12 (sodium/chloride transporters), member 3SLC12A3Sodium ion binding Sodium:chloride
R.GARP(S*)VSGALDPK.AS811YesMS2Basophilicsymporter activity Symporter activity Transporter activity
NP_000329Sodium potassium chloride co-transporter 2SLC12A1Potassium ion binding Sodium ion binding
K.IEYYRN(T*)GSISGPK.VT118NoMS2, MS3N/ASodium:potassium:chloride symporter activity
K.IEYYRNTG(S*)ISGPK.VS120NoMS2, MS3BasophilicSymporter activity Transporter activity
  • a Table contains phosphopeptides found in urinary exosomes. MSn refers to spectra for phosphorylation site identification, Motif refers to phosphorylation motif site and GO Function.