Table 1.

List of phosphopeptides that show significant change in abundance or no significant change with dDAVP treatment compared with control samples

No.Protein NameAccession No.Sequence (Charge)Mean D/CLog2 (D/C)Sites
Part A
1aquaporin 2 (collecting duct)NP_037041R.QSVELHS*PQSLPR.G(+2)0.06−3.95 ± 0.24aS261
2aquaporin 2 (collecting duct)NP_037041R.RQSVELHS*PQSLPR.G(+3)0.10−3.39 ± 0.13aS261
3aquaporin 2 (collecting duct)NP_037041R.QSVELHS*PQSLPR.G(+3)0.11−3.20 ± 0.15aS261
4ArfGAP with FG repeats 1NP_001129068K.SLLGESAPALHLNKGT*PT*QSPVVGR.S(+3)0.34−1.55 ± 0.27aT177,b T179b
5septin 9 isoform 2NP_789826R.LVDTLSQRS*PKPSLR.R(+3)0.41−1.30 ± 0.08aS67b
6ArfGAP with FG repeats 1NP_001129068K.SLLGESAPALHLNKGT*PT-QS*PVVGR.S(+3)0.44−1.19 ± 0.21aT177, S181
7ysv oncogene homolog isoform ANP_110484R.TIYVRDPT*S*NKQQRPVP-ES*QLLPGQR.F(+3)0.50−1.01 ± 0.37cT37,b S38,b S48b
8(ABC1), member 1NP_835196K.HVKAEMEQMALDVGLP-PS*KLK.S(+3)0.50−1.00 ± 0.49cS964
9tensin like SH2 domain containing 1XP_341257R.WDSY*ENMSADGEVLHT-QGPVDGSLYAK.V(+3)0.51−0.98 ± 1.12cY410b
10hsp 90, alpha (cytosolic), class A member 1NP_786937R.DKEVS*DDEAEEKEEK.E(+3)0.52−0.93 ± 0.82cS231
11calpastatin isoform aNP_445747K.NEAITGPLPDS*PKPMGID-HAIDALSSDFTCS*SPTGK.Q(+3)0.55−0.86 ± 1.15cS260,b S280b
12calpastatin isoform aNP_445747K.NEAITGPLPDS*PKPMGID-HAIDALSSDFTCSS*PTGK.Q(+3)0.55−0.86 ± 1.15cS260,b S281b
13calpastatin isoform aNP_445747K.NEAITGPLPDS*PKPMGID-HAIDALSSDFTCSSPT*GK.Q(+3)0.56−0.84 ± 1.13cS260,b T283b
14tensinXP_237286R.HPVGSHQVPGLHSGVVTT-PGS*PSLGR.H(+4)0.60−0.73 ± 0.43cS1523b
15tx elongation factor A (SII) 1 isoform 2XP_001059640K.KKEPAISSQNS*PEAR.E(+3)0.62−0.70 ± 0.77cS100b
16tight junction protein 2NP_446225R.SQEES*PVPQPR.T(+2)0.62−0.69 ± 0.09aS463
17nuclear RNA export factor 3XP_001053711K.S*SNINS*ILELFPK.L(+2)0.62−0.69 ± 0.23aS287,b S292b
18PDLIM1 interacting kinase 1 likeNP_001101454R.LDTS*DLEPT*LKVADFGLS-KVCS*ASGQNPEEPVSVNK.C(+3)0.62−0.68 ± 0.86cS180,b T185,b S198b
19(urea transporter), member 2 isoform 2NP_808877K.LYESELSSPT*WPSSSQDTH-PALPLLEMPEEK.D(+3)0.63−0.67 ± 0.93cT37b
20epsin 3NP_001019962R.TPVLPSGPPITDPWAPSSPT*PK.L(+2)0.63−0.66 ± 0.57cT361b
21(urea transporter), member 2 isoform 2NP_808877K.DLRS*S*DEDSHIVK.I(+3)0.63−0.66 ± 0.59cS62, S63
22tensinXP_237286K.VSS*SPVANGMAS*PSGSST-VSFSHTLPDFSK.Y(+3)0.63−0.66 ± 0.43cS1667,b S1676b
23calnexinNP_742005K.SDAEEDGGTGS*QDEEDSK-PK.A(+3)0.64−0.65 ± 0.59cS563b
24AHNAK nucleoprotein isoform 1XP_001078032K.LEGEIKVPDVDISS*PGVNVE-APDIHVK.A(+4)0.64−0.65 ± 0.19cS1807b
25prostaglandin E synthase 3 (cytosolic)NP_001124461K.DWEDDS*DEDMSNFDR.F(+2)0.64−0.64 ± 1.10cS113b
26beta-2-syntrophinXP_001071043R.GLGPPS*PPAPPR.G(+2)0.64−0.64 ± 0.37cS120
27PC4 and SFRS1 interacting protein 1NP_786941K.NLAKPGVT*STSDS*EEDDD-QEGEK.K(+3)0.66−0.60 ± 1.07cT269,b S274b
28tight junction protein 2NP_446225R.KVQVAPLQGS*PPLSHDDR.G(+3)0.66−0.59 ± 0.24cS107b
Part B
1AHNAK nucleoprotein isoform 1XP_001078032R.NRS*NS*FSDEREFSAPST-PTGTLEFAGGEGK.G(+3)1.510.59 ± 0.25cS2454,b S2456b
2AHNAK nucleoprotein isoform 1XP_001078032R.NRSNS*FS*DEREFSAPSTP-TGTLEFAGGEGK.G(+3)1.510.59 ± 0.25cS2456,b S2458b
3leucine-rich repeat (in FLII) interacting protein 2NP_001019932R.RGSGDTSS*LIDPDTSLSE-LR.E(+3)1.550.63 ± 0.29cS138b
4aquaporin 2 (collecting duct)NP_037041R.RRQS*VELHS*PQSLPR.G(+3)1.550.63 ± 0.06aS256, S261
5tankyrase 1-binding protein of 182 kDaXP_215763K.RAS*VSTNQNTDENDQELR.M(+3)1.670.74 ± 0.18aS883b
6aquaporin 2 (collecting duct)NP_037041R.RQS*VELHS*PQSLPR.G(+2)1.730.79 ± 0.17aS256, S261
7PCTAIRE protein kinase 3NP_001093976R.RFS*MEDLNKR.L(+3)1.770.82 ± 0.05aS66b
8AHNAK nucleoprotein isoform 1XP_001078032R.NRS*NS*FSDEREFSAPSTPT-GTLEFAGGEGK.G(+4)1.910.93 ± 0.41cS2454,b S2456b
9aquaporin 2 (collecting duct)NP_037041R.RRQS*VELHS*PQSLPR.G(+2)1.930.95 ± 0.21aS256, S261
10calcium regulated heat stable protein 1NP_690003R.DRS*PS*PLRGNVVPSPLPTR.R(+3)2.281.19 ± 0.27aS30, S32
11desmoplakin isoform I isoform 2XP_225259R.SMS*FQGIR.Q(+2)2.281.19 ± 0.77cS2216b
12PDZ and LIM domain 5NP_445778R.RGS*QGDIKQQNGPPR.K(+3)2.641.40 ± 0.42aS228
13beta-cateninNP_445809R.RTS*MGGTQQQFVEGVR.M(+3)2.691.43 ± 0.04aS552b
14beta-cateninNP_445809R.RTSMGGT*QQQFVEGVR.M(+3)2.691.43 ± 0.04aT556b
15PCTAIRE protein kinase 3NP_001093976R.RFS*MEDLNK.R(+2)3.051.61 ± 0.21aS66
16beta-cateninNP_445809R.RTS*MGGTQQQFVEGVR.M(+2)3.291.72 ± 0.19aS552b
17aquaporin 2 (collecting duct)NP_037041R.RQS*VELHS*PQS*LPR.G(+3)3.921.97 ± 0.07aS256, S261, S264
18aquaporin 2 (collecting duct)NP_037041R.RRQS*VELHSPQS*LPR.G(+3)4.142.05 ± 0.13aS256,b S264b
19plexin domain containing 2NP_001101892R.RGS*GHPAYAEVEPVGEK-EGFIVSEQC.-(+3)4.382.13 ± 0.24aS507
20aquaporin 2 (collecting duct)NP_037041R.RRQS*VELHSPQSLPR.G(+3)9.003.17 ± 0.20aS256
21aquaporin 2 (collecting duct)NP_037041R.RRQS*VELHS*PQS*LPR.G(+2)9.383.23 ± 0.39aS256, S261, S264
22kinesin 13BNP_998791R.RRS*S*GLQPQGAPEAR.R(+3)10.933.45 ± 0.20aS1718, S1719
23aquaporin 2 (collecting duct)NP_037041R.RQS*VELHS*PQS*LPR.G(+2)12.303.62 ± 0.41aS256, S261, S264
24aquaporin 2 (collecting duct)NP_037041R.RQS*VELHSPQSLPR.G(+2)14.933.90 ± 0.52aS256
25(urea transporter), member 2 isoform 1NP_062220R.RKS*VFHIEWSSIR.R(+4)17.514.13 ± 0.22aS486
26(urea transporter), member 2 isoform 1NP_062220R.RKS*VFHIEWSSIR.R(+3)19.844.31 ± 0.31aS486
  • Mean and SE values are calculated from log2(D/C), where D and C are peak areas for dDAVP-treated and control samples, respectively. Phosphorylation sites are indicated with an asterisk.

  • aP < 0.05; significant change in abundance compared with control samples.

  • bAmbiguity in phosphorylation site assignment by the Ascore algorithm, despite certainty in phosphopeptide identification.

  • cNo significant change in abundance compared with control samples.