Table 6.

Comparison of methods to measure fibrosis

TechniqueRoutine SlideEase (Minutes per Assay)Interassay Reproducibility (R2 for Duplicate Slides)Interobserver Reproducibility (R2)Dynamic Range (% Min-Max Observed)Correlation with Functiona
Sirius Red polarizedNo60High (0.94)n.d.bLow (4 to 22)
Sirius Red unpolarizedNo45High (0.96)n.d.Moderate (12 to 53)+++
Collagen IIINo20Moderate (0.83)High (0.99)High (24 to 74)+++
Trichrome-PASYes40Moderate (0.69)High (0.96)Moderate (38 to 82)+
Visual % fibrous tissueYes5Moderate to high (0.75 to 0.90)Moderate to high (0.75 to 0.99)High (3 to 90)+++
Visual % fibrotic cortexYes5Moderate to high (0.62 to 0.88)Moderate to high (0.75 to 0.93)High (3 to 100)++++
  • aOne + for each functional correlation, P < 0.02.

  • bn.d., not done.