Table 2.

Primers used to quantify human mRNAs in urine samples

Human Primers RT-PCRProduct Length (bp)
MCP-15′-TCG CTC AGC CAG ATG CAA TCA ATG-3′ (sense)250
5′-AGT TTG GGT TTG CTT GTC CAG GTG-3′ (antisense)
NGAL5′-TCT CAG AGA AGA CAA AGA CCC GCA-3′ (sense)321
5′-AGT TCC GAA GTC AGC TCC TTG GTT-3′ (antisense)
GAPDH5′-CTC TTC ACC ACC ATG GAG AAG-3′ (sense)490
5′-GCT TCA CCA CCT TCT TGA TGT CAT-3′ (antisense)
b-actin5-ACC AAC TGG GAC GAC ATG GAG AAA′-3′ (sense)856
5′-ACT CCT GCT TGC TGA TCC ACA TCT-3′ (antisense)
18s5′-CCA GAG CGA AAG CAT TTG CCA AGA-3′ (sense)499
5′-TAT TGC TCA ATC TCG GGT GGC TGA-3′ (antisense)
BRG-15-TCC GGC AGA AGA AAT CAT CAC GGA′-3′ (sense)224
5′-TCC ACT GCT GCT CTC CTT GTA CTT-3′ (antisense)