Table 1.

Comparison of morphometric techniques:a Extent of fibrosis and interassay reproducibility in duplicate sections

TechniqueAssay DayMean (%)SD (%)Minimum (%)Maximum (%)Average Changeb (%)R2c
Sirius Red polarized11354220.9 ± 0.80.94
2135423
Sirius Red unpolarized1311212511.8 ± 1.80.96
231121553
Collagen III1391124609.0 ± 5.00.83
248123274
Trichrome-PAS1641338827.8 ± 3.60.69
259124378
  • aSerial sections stained and evaluated on separate days from 15 biopsies.

  • bMean ± SD of absolute difference between day 1 and day 2 values, 15 sections in each group.

  • cCoefficient of determination between day 1 and day 2 values, all P ≤ 0.0001.