Table 2.

Identification of proteins in HDL isolated from hemodialysis patients (HD) and control subjects (control)

Accession NumberProtein NameHDL-derived PeptidesPRatio
HD (n = 27)Control (n = 19)
178775ApoA-I331.3(27/27)416.2(19/19)<0.0010.8
4502149ApoA-II60.9(27/27)72.3(19/19)<0.0010.8
167887493ApoC-III46.5(27/27)33.5(19/19)<0.0011.4
119588814SAA145.6(27/27)5.6(17/19)<0.0018.2
10835095SAA444.9(27/27)37.9(19/19)0.0011.2
4557325ApoE42.9(27/27)45.0(19/19)0.6711.0
4502027Albumin40.8(27/27)13.5(19/19)<0.0013.0
4502157ApoC-I25.4(27/27)30.2(19/19)0.0010.8
4502163ApoD17.2(27/27)16.2(19/19)0.3001.1
32130518ApoC-II13.9(27/27)8.5(19/19)0.0011.6
22091452ApoM8.0(27/27)11.8(19/19)0.0020.7
105990532ApoB4.0(4/27)(0/19)0.067
114062Apo(a)3.2(5/27)0.4(3/19)0.778
93163358ApoA-IV2.9(27/27)(0/19)<0.001
15080499Antitrypsin2.9(11/27)(0/19)<0.001
18088326RBP42.0(17/27)(0/19)<0.001
114318993Transthyretin1.0(14/27)(0/19)<0.001
6960317α-2 catenin1.0(14/27)0.6(3/19)0.002
298532Paraoxonase 10.8(8/27)0.4(3/19)0.329
34364645Igα0.7(5/27)(0/19)0.067
45643462GIP 120.3(1/27)(0/19)
4557894Lysozyme0.3(1/27)(0/19)
194386720KIAA05900.3(4/27)(0/19)
7020972PHIP0.3(7/27)(0/19)
4503107Cystatin C0.3(5/27)(0/19)
194386720Unknown protein0.3(4/27)(0/19)
4505733Unknown protein0.3(7/27)(0/19)
146424184ApoC-IV0.2(5/27)(0/19)
179665Complement C30.1(1/27)(0/19)
4885179Defensin α30.1(1/27)(0/19)
119579599Haptoglobin0.1(1/27)(0/19)
181482Vit.D-BP0.1(2/27)(0/19)
4507261Statherin0.1(1/27)(0/19)
4505733Platelet factor 4(0/27)0.6(8/19)
148745121ApoL-I(0/27)0.2(1/19)
Mean total peptides/subject698 ± 61693 ± 79
Lp-PLA2 mass (densitometry units)2.7 ± 1.31.00 ± 0.8<0.001
  • HDL was isolated from 27 HD patients and 19 control subjects by one-step ultracentrifugation. The HDL proteome was analyzed on a LC-MS/MS system. The data were analyzed by searching the human NCBI nonredundant public database with Spectrum mill Rev. A0.03.03.078 (Agilent) and Mascot 2.2 (MatrixScience). The values shown represent the mean peptide count per analyzed subject with the corresponding number of patients where respective protein was identified. Statistical significance was calculated with a Mann-Whitney U test. Lp-PLA2 mass associated with HDL was determined by Western blot and densitometric quantification. GIP12, growth-inhibiting protein 12; Vit.D-BP, vitamin D-binding protein; PHIP, PH interacting protein. —, not detected.