Table 5.

Serum concentrations of soluble TNF receptors and flow cytometry analysis of surface expression of TNFα-pathway proteins on peripheral blood cells stratified by group-specific median serum concentration of TNFR2

CharacteristicGroup Stratification (TNFR2 Median)aP Valueb
Low (n=17)High (n=17)
Serum sTNFR2 (pg/ml)2442±2604079±1098By design
Serum sTNFR1 (pg/ml)1310±2762291±773<0.001
Leukocyte counts (×109/L)6.6±1.76.4±1.90.63
 neutrophils4.4±2.04.2±1.30.81
 lymphocytes2.8±4.51.5±0.60.25
 monocytes0.4±0.10.5±0.20.07
Surface expression of TNFα-pathway proteinsc
 TNFα protein on
  neutrophils24±2117±250.24
  T lymphocytes, CD3+1.4±1.81.5±1.80.87
  monocytes24±1725±310.76
 TNFR1 protein on
  neutrophils105±5887±820.24
  T lymphocytes, CD3+2.0±1.71.5±2.80.50
  monocytes135±71121±1040.37
 TNFR2 protein on
  neutrophils27±1024±70.34
  T lymphocytes, CD3+9±69±40.97
  monocytes122±32115±310.48
 ADAM17 protein on
  neutrophils77±6.274±6.60.15
  T lymphocytes, CD3+3.1±1.13.3±1.30.62
  monocytes141±13133±170.12
  • Data are the mean ± SD.

  • a Low and high denote strata below and above median TNFR2 in each group (2769 pg/ml), accordingly.

  • b P value for t test on log-transformed values.

  • c Data are mean fluorescence intensities, the differences between the intensities for the surface marker and the isotype control.