Table 1.

Body weight, food intake, and urinary values of Rhcg+/+, Rhcg+/−, and Rhcg−/− mice during 9 days of HC protein loading

VariableBasal Status2 Days of HC Diet4 Days of HC Diet9 Days of HC Diet
Rhcg+/+ (n=8)Rhcg+/− (n=8)Rhcg−/− (n=8)Rhcg+/+ (n=8)Rhcg+/− (n=8)Rhcg−/− (n=8)Rhcg+/+ (n=8)Rhcg+/− (n=8)Rhcg−/− (n=8)Rhcg+/+ (n=8)Rhcg+/− (n=8)Rhcg−/− (n=8)
Body weight (g)28.2±0.629.7±0.427.0±0.828.0±0.529.5±0.726.0±0.927.6±0.629.0±0.725.7±0.927.8±0.427.3±0.925.9±0.9
Food intake (g/24 h/body wt)0.14±0.020.14±0.030.15±0.020.10±0.02a0.08±0.04a0.10±0.04a0.10±0.02a0.10±0.03a0.10±0.02a0.12±0.02a0.10±0.05a0.10±0.02a
Water intake (g/24 h/body wt)0.16±0.010.14±0.010.15±0.010.24±0.03a0.21±0.02a0.21±0.02a0.22±0.03a0.23±0.02a0.26±0.03a0.25±0.03a0.24±0.03a0.26±0.03a
Urine values
 Volume (ml/24 h)1.89±0.321.91±0.181.74±0.194.51±0.44a4.34±0.29a5.20±0.37a4.24±0.50a4.38±0.38a5.523±0.55a4.52±0.17a4.36±0.24a5.65±0.30a,b
 Urinary pH5.94±0.085.97±0.046.03±0.066.0±0.045.94±0.035.87±0.045.97±0.046.03±0.055.89±0.045.91±0.045.85±0.045.86±0.07
 NH4+/urine volume (mmol/24 h)0.028±0.010.028±0.010.027±0.010.26±0.08a0.24.0±0.07a0.16±0.04a,c0.29±0.08a0.24±0.07a0.17±0.05a,d0.28±0.08a0.28±0.05a0.29±0.08a
 TA/urine volume (mmol/24h)0.08±0.060.09±0.030.07±0.030.22±0.09a0.25±0.05a0.22±0.06a0.20±0.08a0.23±0.06a0.23±0.08a0.24±0.07a0.28±0.06a0.27±0.05a
 Ca2+/urine volume (μmol/24 h)1.5±0.11.6±0.11.2±0.13.3±0.1a3.2±0.7a6.5±0.2a,b5.4±0.3a5.5±0.2a8.2±0.4a,c4.1±0.46.8±0.2a5.1±0.4a
 Dpd/urine volume (nmol/24 h)0.076±0.04 (n=6)ND0.073±0.02 (n=6)NDNDND0.100±0.07 (n=6)ND0.211±0.01a,c (n=6)0.093±0.02 (n=6)ND0.149±0.05a,c (n=6)
  • Body weight, food intake, and urinary values of Rhcg+/+, Rhcg+/−, and Rhcg−/− mice during 9 days HC protein loading (n=8 mice for each group). ND, not determined.

  • a P≤0.05 significantly different from same genotype under control conditions.

  • b P≤0.01 significantly different from Rhcg+/+ mice under same treatment conditions.

  • c P≤0.05 significantly different from Rhcg+/+ mice under same treatment conditions.

  • d P≤0.001 significantly different from Rhcg+/+ mice under same treatment conditions.