Table 1.

Concentrations, affinity, and IgG subclasses of antibodies to PLA2R

PatientsELISA (U/ml)Concentration (µM)KD (M) IgG1 (%)IgG2 (%)IgG3 (%) IgG4 (%)
130,0001.4±0.060.49×10−10169272
226000.32±0.10.45×10−10451045
330,0001.0±0.120.43×10−101027063
423000.36±0.190.85×10−101110078
593000.51±0.180.32×10−1017221942
  • Absolute concentrations of PLA2R-specific antibody detected in five patient sera tested by ELISA (U/ml) and derived from three kinetic runs (µM). The KD column is the kinetic constants for the binding of patient autoantibodies to immobilized N-C3. The last four columns indicate the percentage of each IgG subclass (IgG1–4) in the five patient sera measured by ELISA,5 demonstrating the presence of predominantly IgG4. Values expressed with a plus/minus sign are the mean±SD.