Table 1.

Cystinosin-interacting proteins

Accession UniProtaAccession NCBIbGene NameProtein DescriptionCystinosinaCystinosinbN288AaN288KaN288KbN323KbK280RbΔYFPQAbΔYMNFbΔGYDQLb2mbCD63bLamp1b
Experiment No.123412341112111112121212
Human Cystinosin-EGFP3257224540484547201750667642514930272831
P50516gi57109638ATP6V1AV-type proton ATPase catalytic subunit A1933253911244028261363614303021
P62814gi73993818ATP6V1B2V-type proton ATPase subunit B, brain isoform21232333111935282423532443031412
Q9Z1G3gi73974252ATP6V1C1V-type proton ATPase subunit C 16128193319971917781
P57746gi57090225ATP6V1DV-type proton ATPase subunit D26474551107177
P50518gi57106611ATP6V1E1V-type proton ATPase subunit E 161261248148511081813
Q9D1K2gi57095928ATP6V1FV-type proton ATPase subunit F824123
Q9CR51gi345777909ATP6V1G1V-type proton ATPase subunit G 12222413321
Q8BVE3gi73999099ATP6V1HV-type proton ATPase subunit H48514410169817141168
Q9R1Q9gi74008854ATP6AP1V-type proton ATPase subunit S11322935321341
P51863gi73957263ATP6V0D1V-type proton ATPase subunit d 110109181112181412111171343141713
P63082gi57088089ATP6V0CV-type proton ATPase 16 kDa proteolipid subunit613411
Q9Z1G4gi57100281ATP6V0A1V-type proton ATPase 116 kDa subunit a isoform 1172010221414181310121191213
P15920gi73965740ATP6V0A2V-type proton ATPase 116 kDa subunit a isoform 221415221442217469
Q9CQ22gi73988027LAMTOR1Ragulator complex protein LAMTOR143511143
Q9JHS3gi57089103LAMTOR2Ragulator complex protein LAMTOR231
O88653gi74002014LAMTOR3Ragulator complex protein LAMTOR321111
Q9D1L9gi545502881LAMTOR5Ragulator complex protein LAMTOR51
Q99K70gi345780494RRAGCRas-related GTP-binding protein C345105215
Q80×95gi359320734RRAGARas-related GTP-binding protein A2163122
  • Proteins identified by mass spectrometry from co-IP experiments with cystinosin-EGFP and its mutants, as well as EGFP-CD63 and Lamp1-EGFP controls, using GFP antibodies are reported for 3T3 and MDCK cells. The numbers represent the total number of peptides used for the identification of the respective protein. The accession numbers according to the database used are described. All biologic replicates (1–4) were performed at different times always in parallel with nontransduced cells as internal controls (not shown, as all values were null).

  • a 3T3 cells.

  • b MDCK cells.