Table 1.

CRISPR/Cas9 resources

Resource NameDescriptionWeb Address
Benchling-crisprHighly integrated sgRNA design tool that supports ≥40 reference genomes; automatically annotates genome coordinates with the exon and coding DNA sequencehttps://benchling.com/crispr
Optimized CRISPR design, Zhang laboratory, Massachusetts Institute of TechnologyInput sequence region of interest sequence and software lists candidate sgRNA sequences with scores on the basis of the number of off targets and positions of mismatches; 16 different specieshttp://crispr.mit.edu/
E-CRISP, Boutros laboratory, German Cancer Research CenterHighly customizable sgRNA calculator including efficiency and possible off–target siteshttp://www.e-crisp.org/E-CRISP/
CRISPRdirect, Database Center for Life Sciences, JapanSearch for sgRNAs by gene accession number, genomic location, or sequence; >200 species and sgRNA priority on the basis of the number of off-target siteshttp://crispr.dbcls.jp/
The CRISPR page at CNBA compendium of landmark articles, background information, and links to other CRISPR resourceshttp://wwwuser.cnb.csic.es/~montoliu/CRISPR/
Google GroupA forum for real-time discussion of all topics related to CRISPR/Cas; currently, 2290 topics and 3959 membershttps://groups.google.com/forum/#!forum/crispr
Addgene’s CRISPR/Cas9 Plasmids and ResourcesExcellent overview of CRISPR/Cas9 and the best place to acquire all plasmids needed for gene editinghttps://www.addgene.org/crispr/
  • As of May of 2016.