Table 1.

Major moss N-glycan structures and corresponding calculated mass-to-charge ratios of FH glycopeptides

Glycan Structure AA 792–808 LCPPPPQIPNSHNMoxTTT [M+4H+]AA 850–867 IPCSQPPQIEHGTINSSR [M+4H+]AA 889–901 ISEENETTCYMGK [M+3H+]AA 889–901 ISEENETTCYMoxGK [M+3H+]AA 1006–1018 MDGASNVTCINSR [M+3H+]AA 1006–1018 MoxDGASNVTCINSR [M+3H+]
Man4744.5715769.5947872.6785878.0101827.0034832.3351
MGn754.8282779.8513886.3540891.6857840.6789846.0106
Man5785.0847810.1079926.6961932.0277881.0210886.3527
Man4Gn947.6509972.67411075.75791081.08951030.08281035.4145
GnGna805.5980830.6212954.0471959.3788908.3721913.7037
Man6825.5979850.6211980.7137986.0453935.0386940.3703
M(AF)b831.8559856.8790989.0576994.3892943.3825948.7142
Man5Gn835.8546860.8777994.3892999.7209948.7141954.0458
GnGnXb937.6403962.66351086.08941091.42111040.41441045.7460
GnGnFb951.6559976.67911100.10501105.43671054.43001059.7616
Man7866.1111891.13431034.73131040.0629989.0562994.3879
GnGnXFb1083.69821108.72141232.14731237.47901186.47231191.8039
Gn(AF)b882.6257907.64891056.75071062.08241011.07561016.4073
Man8906.6243931.64751088.74891094.08051043.07381048.4055
  • An oxidized methionine is labeled with Mox, and the charge state of a glycopeptide is shown by [M+nH+]. AA, amino acid; MGn, terminal mannose on one antenna.

  • a The identified main glycopeptides in MS analysis of glyco-engineered moss-produced FH.

  • b Glycopeptides displaying core fucose and xylose were not detected.