Table 1.

Relative anion permeability measured in HEK cells

MutantsP(Br)/P(Cl)P(NO3)/P(Cl)P(SCN)/P(Cl)
ClC-Ka (wild type)0.84±0.020.25±0.042.75±0.52
S121T0.93±0.050.26±0.032.35±0.24
S121G0.98±0.060.31±0.045.58±1.20
S121P1.09±0.05a0.29±0.033.59±0.51
Y425A1.00±0.03a0.45±0.05a1.99±0.30
  • Relative permeabilities to bromide, nitrate, and thiocyanate in respect to chloride for wild-type ClC-Ka and the mutants S121T, S121G, S121P, and Y425A measured from patch-clamp experiments in HEK cells. Values were obtained from the shift in the reversal potential of the currents as described in the Methods. Data are presented as the mean ± SEM.

  • a Indicates a significant difference (P<0.01).