Table 1.

Summary of characterization for all urine cells reprogrammed to pluripotency in the course of this study and the resulting urinary induced pluripotent stem cell clones

Urine Cells
C0406S0730LY0815LXY0828NS0816XB0722WXM0816ZGZ0816CGK0828XJY0903ZQ0907LBJ0828GM1020
Female, 24 years, CaucasianFemale, 24 years, CaucasianFemale, 25 years, AsianFemale, 27 years, AsianFemale, 28 years, AsianMale, 27 years, AsianMale, 25 years, AsianMale, 27 years, AsianMale, 24 years, AsianMale, 28 years, AsianMale, 26 years, AsianMale, 27 years, AsianFemale, 65 years, Asian
Volume of urine100 ml100 ml150 ml140 ml160 ml50 ml100 ml60 ml90 ml200 ml100 ml100 ml100 ml
Major cell typeType 1Type 2Type 1Type 2Type 1Type 2Type 2Type 2Type 1Type 2Type 2Type 1Type 1
    qPCR
    IFNDNDNDNDNDND
Passage of UC for reprogrammingP2P2P3P2P2P2P3P2P3P2P3P3P2
Reprogramming efficiency0.30%4%0.10%0.30%3%0.10%0.30%3%0.20%2%0.20%0.20%0.01%
Number of UiPSC colonies picked41122522322222
UiPSC characterization
    qPCR4/45/112/22/23/52/22/23/32/22/22/22/21/2
    IF2/42/11NDNDND1/2NDND1/2NDNDNDND
    DNA methylation2/42/111/21/21/52/22/21/32/21/21/21/21/2
    Karyotype1/41/111/21/21/51/21/21/31/21/21/21/21/2
    DNA microarrays1/41/11NDNDNDNDNDNDNDNDNDNDND
    STR analysis1/41/111/21/21/51/21/21/31/21/21/21/21/2
Differentiation
    EBs2/42/11NDNDND1/2NDNDNDNDNDNDND
    neurons2/41/111/21/21/51/21/21/3NDNDNDNDND
    cardiomyocyes2/42/111/21/22/51/21/21/31/21/2ND1/2ND
    hepatocytes2/41/11NDNDNDNDNDNDNDNDNDNDND
    teratomas2/41/4NDNDNDNDNDNDNDNDNDNDND
  • Quantitative real-time PCR (qPCR) and immunofluorescence (IF) analysis are routinely included all markers in Figures 1 and 2. Reprogramming efficiency refers to alkaline phosphatase-positive clones with human embryonic stem cell (ESC) morphology. Neuronal differentiation for urinary induced pluripotent stem cells (UiPSC) clones not mentioned in Figure 3 was only assessed morphologically; as for cardiomyocyte differentiation, we only verified the generation of beating clusters. √, done; UC, urine cells; STR, single tandem repeat; EBs, erythroid bodies.